Everyone has/knows matrigel. It's the "3D" culture vessel of choice; which is basically a cocktail of growth factors and basement membrane adherent factors that promote cell growth in non adherent conditions and preventing apoptosis.
We want to study how the tumor grows and how we can get a targeted drug inside that tumor. In vitro, we use siRNA technology to "knock down" the protein e.g., to prevent the protein's function to study it's biology in it's absence. In 3D cell culture this allows us to recreate mini microenvironments to test new drugs.
A 3D cell culture spheroid using matrigel.
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