The BIACORE is an optical biosensor that uses surface plasmon resonance (SPR) for real-time monitoring of macromolecular interactions. (SPR is an light phenomenon that's used to measure changes in solution concentration of molecules). Applications include affinity measurements, binding kinetics, active concentrations, binding specificity and epitope mapping. The technology is for use in drug discovery, antibody screening, ligand fishing and therapeutics. Technical advantages include minimal sample consumption, recovery of surface bound samples for MS analysis, multi-sample analysis and measurement of weak binding events. Typically, protein or DNA is bound by one of several possible methods onto a carboxymethylated dextran-gold surface. The interacting protein of interest is injected over the surface and the kinetics of binding are measured in real time.
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